Solve all of Subject 16's puzzles.
Score 750 points or more on a single kill (Multiplayer Only).
Kill your target while hidden in a hay bale (Multiplayer Only).
Collect All Feathers.
Jump with the parachute off the top of Castel Sant'Angelo.
Complete 10 Guild Challenges.
Train a recruit up to the Rank of Assassin.
Kill a guard with a broom.
Win the highest bet at the Fights.
Kill your target and escape your pursuer in less than 10 seconds (Multiplayer Only).
Burn All Borgia Towers.
Discover the Shrine in the Basilica di San Pietro.
Secure the Apple of Eden.
Complete DNA Sequence 9.
Complete DNA Sequence 8.
Complete DNA Sequence 7.
Discover the Shrine in Palazzo Laterano.
Achieve 100% Synchronization in any Sequence other than Sequence 1.
Discover the Shrine in the Cloaca Maxima.
Destroy the Naval Cannon.
Complete DNA Sequence 6.
Destroy the Tank.
Destroy the Bomber.
Find 1 Artifact in 2012.
Complete DNA Sequence 5.
Complete DNA Sequence 4.
Destroy the Machine Gun.
Recruit 3 Assassins.
Complete DNA Sequence 3.
Renovate 5 buildings in the Antico district.
Discover the Shrine in the Catacombe di Roma.
Throw money in a well.
During Open Conflict, kill your target and escape (Multiplayer Only).
Perform an Execution Streak of 10 kills.
Replay a Memory.
Access your email in 2012.
Complete an entire Session with at least one kill (Multiplayer Only).
Earn 3 gold medals in the Animus Virtual Training Program.
Find a secure place to hide and re-enter the Animus.
Complete DNA Sequence 1.
Attempt to access DNA Sequence 9 for the first time.
Discover the Shrine in the Terme di Traiano.
Discover the Shrine in Il Colosseo.
Remove All Borgia Flags in Rome.
Complete DNA Sequence 2.
Take the lead 10 seconds before the end of a session and go on to win (Multiplayer Only).
Reach Level 50 (Multiplayer Only).
Get all the Co-op bonuses in 1 session (Multiplayer Only).
Get every single bonus at least once (Multiplayer Only).
Perform a double or triple escape (Multiplayer Only).
Throw a Long Weapon, Heavy Weapon, and Smoke bomb more than 10 m at a guard.
Win 10000 florins playing Hazard.
Receive 100% synch in ACB and full synch in all missions of the Da Vinci Disappearance.
Kill 10 guards with a single arrow storm.
Complete the Da Vinci Disappearance.
Beat up the 5 thirsty harlequins.
Double Assassinate from a parachute.
Steal 5 horses from their riders, while remaining on horseback.
Kill a guard with the bag that drops from a lift.
Pay 500 florins to an orator, then pickpocket him afterward.